Journal: Frontiers in Cell and Developmental Biology
Article Title: Proteomic analysis and functional validation reveal distinct therapeutic capabilities related to priming of mesenchymal stromal/stem cells with IFN-γ and hypoxia: potential implications for their clinical use
doi: 10.3389/fcell.2024.1385712
Figure Lengend Snippet: Human amnion-derived mesenchymal stromal/stem cells (hAMSCs) were grown as monolayers with or without priming. (A) Representative DIC images of hAMSCs grown as monolayers without priming (hAMSCs), cultured under hypoxic conditions (hAMSCs after hypoxia), or treated with IFN-γ (hAMSCs after IFN-γ treatment). (B) Representative images of flow cytometry analysis for quantification of hAMSCs at step 2 for both positive (CD90, CD73 and CD13) and negative surface markers (HLA-DR and CD45). Green represents the isotype control, and red represents stained cells. (C) Experimental plan and exosome characterization (size and concentration). DIC, differential interference contrast.
Article Snippet: For phenotypic characterization, hAMSCs were first washed twice with FACS buffer (PBS containing 0.3% BSA and 0.1% NaN 3 ) and then incubated on ice for 30 min with specific antibodies against CD90, CD73, CD13, CD45, and HLA-DR (BD Biosciences, United States).
Techniques: Derivative Assay, Cell Culture, Flow Cytometry, Staining, Concentration Assay